CTS™ StemScale™ PSC Suspension Medium

CTS (Cell Therapy Systems) StemScale PSC Suspension Medium is a xeno-free medium that enables robust expansion of pluripotent stem cells (PSCs) in suspension for translational and clinical applications. CTS StemScale medium is scalable and easy-to-use, utilizing a spheroid self-assembly method to promote growth of multiple PSC lines without the use of microcarriers. PSCs expanded in CTS StemScale medium maintain pluripotency and genomic stability across multiple passages and can be differentiated to the three germ layers.

CTS StemScale PSC Suspension Medium:

• Supports maximal expansion capability versus other PSC suspension media, helping reduce manufacturing time and cost
• Enables scalable expansion of PSCs across multiple culture vessel formats and sizes
• Utilizes a simplified workflow with no cell strainer
• Facilitates regulatory filings with GMP manufacturing, traceability of raw materials, and regulatory documentation

Maximal expansion capability

PSCs cultured in CTS StemScale medium can achieve 5–10-fold expansion per passage and up to 30% higher expansion versus other PSC suspension media. Long-term expansion of PSCs in CTS StemScale medium (>30 passages) can be accomplished without the loss of expansion potential, pluripotency, or genomic stability.

Scalable expansion

CTS StemScale medium promotes consistent growth of PSCs in plates, flasks, and bioreactors. PSCs can be efficiently expanded in CTS StemScale medium across vessel types using the same culture parameters at both small and large scales.

Simplified workflow

CTS StemScale medium promotes spheroid nucleation of both induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). PSCs grown in CTS StemScale medium are fed by a 50% medium replacement strategy. This method reduces metabolic waste build-up while maintaining consistent media volumes in the culture, a critical factor in the maintenance of spheroid size. During passaging, the use of cell strainers is not required when dissociating the PSC spheroids, thereby helping minimize cell loss, improve cell viability, reduce contamination risk, and simplify scale-up to large vessels with high culture volumes.

GMP manufactured

Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing site is ISO 13485-certified. Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available for CTS StemScale PSC Suspension Medium.