Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen™

Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
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货号产品名称数量产品类型
A72128Lyo-ready RPA Kit500 ReactionsDNA Amplification Kit
A72123Lyo-ready T4 Gene 32 Protein1600 μgStand-alone enzyme
A72124Lyo-ready T4 UvsX Protein120 μgStand-alone enzyme
A72125Lyo-ready T4 UvsY Protein120 μgStand-alone enzyme
A72126RPA Reaction Buffer (2X)2000 μLReaction Buffer
A72127Lyo-ready RPA Kit100 ReactionsDNA Amplification Kit
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货号 A72128
价格（CNY)/ Each
-
产品名称:
Lyo-ready RPA Kit
数量:
500 Reactions
产品类型:
DNA Amplification Kit
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
For Research Use Only. Not for use in diagnostic procedures.
规格
产品类型DNA Amplification Kit
形式Liquid
适用于（应用）RPA, RT-RPA
聚合酶Lyo-ready Bst DNA Polymerase
数量500 Reactions
研究类别Nucleic Acid Amplification
最佳反应温度42°C
产品名称Lyo-ready RPA Kit
逆转录酶SuperScript IV Reverse Transcriptase
运输条件Dry Ice
检测方法End point, real-time, lateral flow
反应时间Up to 20 min.
Unit SizeEach
内容与储存
Five boxes, each box contains: Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL, Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL, Lyo-ready Bst DNA Polymerase (6 μg/mu;L), 50 μL, Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL, 2X RPA Reaction Buffer, 1000 μL, 280 mM MgCl2, 100 μL, 10 mM dNTP Mix, 50 μL, Store at -25°C to -15°C.