在高内涵筛选 (HCS) 测定中,可使用多功能 HCS NuclearMask Blue、Red 和 Deep Red 染色剂轻松识别细胞核,这些染色剂可经受基于甲醛的标准固定和基于去垢剂的透化方法,并可应用于活细胞和固定细胞。HCS NuclearMask 染色剂的染料易于与其他荧光基团区分,可用于多参数分析。它们还可以标记整个细胞(即细胞质和细胞核),并提供准确的背景,据此可评估感兴趣的特点。
多功能 HCS NuclearMask Blue、HCS NuclearMask Red 和 HCS NuclearMask Deep Red 染色剂可用于在高内涵成像和分析 (HCS) 平台上对甲醛固定细胞进行 DNA 含量测定和细胞分界。HCS NuclearMask Blue、HCS NuclearMask Red 和 HCS NuclearMask Deep Red 染色剂在与 DNA 结合后,最大激发/发射波长分别为 355/460 nm、622/645 nm 和 638/686 nm。HCS NuclearMask Blue 染色剂可轻松区分可见和近红外波长荧光基团,HCS NuclearMask Red 染色剂可轻松区分绿色甚至橙色荧光团,HCS NuclearMask Deep Red 染色剂可轻松区分可见波长荧光基团,用于多参数分析。
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Abstract
Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging.
Authors:Orsinger GV, Watson JM, Gordon M, Nymeyer AC, de Leon EE, Brownlee JW, Hatch KD, Chambers SK, Barton JK, Kostuk RK, Romanowski M,
Journal:
PubMed ID:24676382
'Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of ... More
The intraviral protein interaction network of hepatitis C virus.
Authors:Hagen N, Bayer K, Rösch K, Schindler M,
Journal:
PubMed ID:24797426
'Hepatitis C virus (HCV) is a global health problem and one of the main reasons for chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The HCV genome is translated into a polyprotein which is proteolytically processed into 10 viral proteins. The interactome of the HCV proteins with the host ... More
Detection of intracellular glutathione using ThiolTracker violet stain and fluorescence microscopy.
Authors:Mandavilli BS, Janes MS,
Journal:Curr Protoc Cytom
PubMed ID:20578109
Glutathione plays an important role in protecting mammalian cells from oxidative stress and cell death. Because reduced glutathione (GSH) represents the large majority of intracellular free thiols, cell-permeant, thiol-reactive fluorescent probes represent potentially useful indicators of intracellular GSH. The ThiolTracker Violet stain (a registered trademark of Invitrogen) is a bright ... More
CD40L induces functional tunneling nanotube networks exclusively in dendritic cells programmed by mediators of type 1 immunity.
The ability of dendritic cells (DC) to mediate CD4(+) T cell help for cellular immunity is guided by instructive signals received during DC maturation, as well as the resulting pattern of DC responsiveness to the Th signal, CD40L. Furthermore, the professional transfer of antigenic information from migratory DC to lymph ... More