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Invitrogen
O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (RL2)
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FIGURE: 1 / 95
O-linked N-acetylglucosamine (O-GlcNAc) Antibody (MA1-072) in WB
Western blot analysis of mouse cortical brain lysates using O-Linked N-Acetylglucosamine Monoclonal Antibody (Product # MA1-072). Blots containing cortical extracts from 4 individual C57BL/6 mice (Lanes 1-4) were blocked with 5% milk in TBST, and probed with MA1-072 (1:1000), followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody. Data courtesy of the Innovators Program.
Product Details
MA1-072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Amphibian,
Bovine,
Chemical,
Fish,
Fruit fly,
Hamster,
Human,
Invertebrate,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RL2
Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues.
MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus.
The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.
Target Information
O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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