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Application
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POLR2C Antibody (MA5-42940) in IHC (P)
Immunohistochemistry analysis of POLR2C in paraffin-embedded human lung cancer. Samples were incubated with POLR2C Monoclonal antibody (Product # MA5-42940) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-42940
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3T8K8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPB3/POLR2C (P19387).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.37 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: 293T, Mouse kidney, Rat lung, Rat brain, Rat spleen.
Immunogen sequence: MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase (RNA) II subunit C; polymerase II polypeptide C; RNA polymerase II 3; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB31; RPB33
Gene Aliases: 33kDa; A-152E5.7; hRPB33; hsRPB3; mRBP31; POLR2C; RPB3; RPB31; Rpo2-3
UniProt ID: (Human) P19387, (Mouse) P97760
Entrez Gene ID: (Human) 5432, (Rat) 361365, (Mouse) 20021
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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