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Invitrogen

PKC alpha/beta/gamma Recombinant Rabbit Monoclonal Antibody (24GB885)

View all (2) PKC alpha/beta/gamma antibodies
Datasheet
Protocols
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Datasheet
Protocols
Tech Support

Product Details

MA5-57173

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Flow Cytometry (Flow)

1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

24GB885

Immunogen

A synthesized peptide derived from human PKC (aa 300-500)
View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Dry ice (international)

Target Information

Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; PKA C-alpha; PKC-B; PKC-beta; PKC-gamma; protein kinase A; protein kinase A catalytic subunit; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; Protein kinase C gamma type; protein kinase C type I (gamma type); protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, gamma; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit

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Gene Aliases: A130082F03Rik; Cs-PKA; PKACA; PKC; PKC-Beta; PKC-gamma; PKCA1; PKCB; PKCC; PKCD; PKCG; PKCgamma; PKCI; PPNAD4; PRKACA; Prkc; PRKCB; PRKCB1; PRKCB2; Prkcc; PRKCG; RATPKCI; SCA14

UniProt ID: (Human) P17612, (Human) P05771, (Human) P05129, (Mouse) P05132, (Mouse) P68404, (Rat) P68403, (Mouse) P63318, (Rat) P63319

Entrez Gene ID: (Human) 5566, (Human) 5579, (Human) 5582, (Rat) 25636, (Mouse) 18747, (Mouse) 18751, (Rat) 25023, (Mouse) 18752, (Rat) 24681

Function(s)
protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding protein complex binding protein kinase A regulatory subunit binding chromatin binding protein kinase C activity calcium-dependent protein kinase C activity protein kinase C binding calcium channel regulator activity zinc ion binding ligand-dependent nuclear receptor transcription coactivator activity histone kinase activity (H3-T6 specific) histone binding androgen receptor binding protein kinase activity Rab GTPase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
G2/M transition of mitotic cell cycle mesoderm formation neural tube closure regulation of heart rate stimulatory C-type lectin receptor signaling pathway renal water homeostasis mRNA processing protein phosphorylation blood coagulation positive regulation of cell proliferation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation activation of protein kinase A activity calcium-mediated signaling using intracellular calcium source lipoprotein metabolic process regulation of protein binding regulation of cellular respiration regulation of osteoblast differentiation protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation negative regulation of meiotic cell cycle regulation of cytosolic calcium ion concentration regulation of synaptic transmission, glutamatergic regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity regulation of proteasomal protein catabolic process regulation of protein processing positive regulation of cell cycle arrest cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning regulation of cardiac conduction regulation of bicellular tight junction assembly response to hypoxia adaptive immune response transcription, DNA-templated regulation of transcription from RNA polymerase II promoter calcium ion transport cellular calcium ion homeostasis apoptotic process mitotic nuclear envelope disassembly signal transduction negative regulation of glucose transport platelet activation positive regulation of vascular endothelial growth factor receptor signaling pathway histone H3-T6 phosphorylation intracellular signal transduction B cell activation lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to carbohydrate stimulus chemical synaptic transmission learning or memory chemosensory behavior phosphorylation negative regulation of protein ubiquitination regulation of response to food positive regulation of mismatch repair negative regulation of protein catabolic process regulation of circadian rhythm response to morphine negative regulation of neuron apoptotic process response to pain rhythmic process regulation of phagocytosis innervation negative regulation of proteasomal protein catabolic process modulation of synaptic transmission immune system process regulation of transcription, DNA-templated regulation of dopamine secretion chromatin modification regulation of growth response to drug response to glucose dibenzo-p-dioxin metabolic process response to vitamin D positive regulation of odontogenesis of dentin-containing tooth response to ethanol
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