EpiLife™ 培养基,含 60 μM 钙
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EpiLife™ 培养基,含 60 μM 钙
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EpiLife™ 培养基,含 60 μM 钙

EpiLife 是一种使用 60 μM 氯化钙制备的无菌液体培养基,适用于人表皮角质形成细胞和人角膜上皮细胞的长期无血清培养。该基础培养基要求在使用前添加适当的生长添加剂。与其他无血清培养基系统相比,EpiLife 培养基可大幅延长人表皮角质形成细胞的体外寿命。该基础培养基不含动物来源成分,并且包含必需和非必需氨基酸、维生素了解更多信息
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货号数量
MEPI500CA500 mL
货号 MEPI500CA
价格(CNY)
1,705.00
Each
-
添加至购物车
数量:
500 mL
物流信息
常规产品: 发货后, 北上广深及省会城市通常为 1-2天,二线城市通常 2-3天,三线以及偏远地区通常 4天,周末以及节假日顺延。
空运受限产品: 发货后, 北上广深及省会城市通常为 2-4天,二线城市 通常3-5天,三线以及偏远地区通常 6-10天,周末以及节假日顺延。
现货中心: 此类产品将由您身边的现货中心极速配送。
价格(CNY)
1,705.00
Each
添加至购物车
EpiLife 是一种使用 60 μM 氯化钙制备的无菌液体培养基,适用于人表皮角质形成细胞和人角膜上皮细胞的长期无血清培养。该基础培养基要求在使用前添加适当的生长添加剂。与其他无血清培养基系统相比,EpiLife 培养基可大幅延长人表皮角质形成细胞的体外寿命。该基础培养基不含动物来源成分,并且包含必需和非必需氨基酸、维生素、其他有机化合物、痕量矿物质和无机盐。它不含抗生素、抗真菌剂、激素、生长因子或蛋白质。EpiLife 培养基使用 HEPES 和碳酸氢盐作为缓冲剂,设计用于在含有 5% CO2 和 95% 空气的培养箱中使用。

为了支持人角质形成细胞的铺板和长期增殖,EpiLife 培养基必须补充选定的激素和生长因子。本公司提供一系列可用于培养人角质形成细胞的生长添加剂。请参阅 EpiLife 培养基用户指南以确定适合您研究目的的理想添加剂。这些添加剂含有人角质形成细胞在 EpiLife 培养基中生长所需的全部生长因子、激素和提取物。
仅供科研使用。不可用于诊断程序。
规格
细胞类型角质化细胞、上皮细胞
无机盐
产品线Cascade Biologics™、EpiLife™
产品类型EpiLife 培养基
数量500 mL
运输条件室温
分类非动物源性, 化学成分明确, 无蛋白, 无血清
培养类型哺乳动物细胞培养
形式液体
血清水平无血清
无菌无菌过滤
灭菌方法无菌过滤
加有添加剂HEPES:, 酚红
Unit SizeEach
内容与储存
请避光冷藏 (2–8°C) 储存。

常见问题解答 (FAQ)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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