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Citations & References (25)
Invitrogen™
pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assaypHrodo™ Red dye conjugatesRead more
| Catalog Number | Quantity |
|---|---|
| P36600 | 1 mg |
Catalog number P36600
Price (CNY)
10,381.00
Each
Quantity:
1 mg
Price (CNY)
10,381.00
Each
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay
pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes
Get faster staining and more accurate results - without the need for wash steps or quencher dye
• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to the acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo™ Red E.coli BioParticles™ conjugates in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
Consult user Manual for solubility instructions.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Emission587 nm
Excitation560 nm
Label or DyepHrodo™ Red
Molecular Weight (g/mol)∼650
Product TypeSuccinimidyl Ester
Quantity1 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
ColorRed
Label TypeClassic Dyes
Product LinepHrodo
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C) and protect from light.
Citations & References (25)
Citations & References
Abstract
Tim4- and MerTK-mediated engulfment of apoptotic cells by mouse resident peritoneal macrophages.
Journal:
PubMed ID:24515440
'Apoptotic cells are swiftly engulfed by macrophages to prevent the release of noxious materials from dying cells. Apoptotic cells expose phosphatidylserine (PtdSer) on their surface, and macrophages engulf them by recognizing PtdSer using specific receptors and opsonins. Here, we found that mouse resident peritoneal macrophages expressing Tim4 and MerTK are
The effect of endosomal escape peptides on in vitro gene delivery of polyethylene glycol-based vehicles.
Journal:J Gene Med
PubMed ID:18642401
'BACKGROUND: With recent progress in gene therapy clinical trials, there is an even greater demand to advance the development of nonviral gene delivery vehicles. We have previously developed poly(ethylene glycol) (PEG)-based vehicles with transfection efficiency similar to polyethyleneimine and low cytotoxicity. It was hypothesized that conjugating endosomal escape peptides (EEPs)
Initial cytoplasmic and phagosomal consequences of human neutrophil exposure to Staphylococcus epidermidis.
Journal:Cytometry A
PubMed ID:19937952
'Microorganisms are recognized by specific phagocyte surface receptors. Liganded receptors then signal a series of events leading to phagocytosis and destruction of the organism by oxidative, lytic, and associated processes. Some organisms, such as Mycobacterium tuberculosis (Mtb), Cryptococcus neoformans (Cf), and others, evade such destruction, surviving and sometimes multiplying within
Zebrafish as a novel vertebrate model to dissect enterococcal pathogenesis.
Journal:
PubMed ID:24002065
'Enterococcus faecalis is an opportunistic pathogen responsible for a wide range of life-threatening nosocomial infections, such as septicemia, peritonitis, and endocarditis. E. faecalis infections are associated with a high mortality and substantial health care costs and cause therapeutic problems due to the intrinsic resistance of this bacterium to antibiotics. Several
Engulfing Astrocytes Protect Neurons from Contact-Induced Apoptosis following Injury.
Journal:PLoS One
PubMed ID:22461890
'Clearing of dead cells is a fundamental process to limit tissue damage following brain injury. Engulfment has classically been believed to be performed by professional phagocytes, but recent data show that non-professional phagocytes are highly involved in the removal of cell corpses in various situations. The role of astrocytes in