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Application
FIGURE: 1 / 1
Phospho-IGF1R beta (Tyr1316) Antibody (PA5-78017) in WB
Western Blot using Phospho-IGF1R beta (Tyr1316) Polyclonal Antibody (Product # PA5-78017). Untreated (–) and treated (+) MCF-7 whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1346) antibody Phospho-IGF1R beta (Tyr1316) Polyclonal Antibody (Product # PA5-78017) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect t... View More
Product Details
PA5-78017
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IGF1R (phospho Tyr1316). The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.83 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736384
Product Specific Information
Positive Control: IGF-1 treated MCF-7 cell
Predicted Reactivity: Xenopus laevis (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD221; IGF-I receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2
Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
protein tyrosine kinase activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
protein binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
transmembrane signal receptor
immune response
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of DNA replication
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
protein tetramerization
inactivation of MAPKK activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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