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引用和文献 (64)
Invitrogen™
TC-FlAsH™ II 细胞内四半胱氨酸标记检测试剂盒（绿色荧光），用于活细胞成像
TC-FlAsH™ II 细胞内四半胱氨酸标记检测试剂盒包含一种用于活细胞标记的基于表达标记的荧光标记试剂。可使用试剂盒中包含的绿色荧光 FlAsH-EDT2 试剂标记表达与四半胱氨酸短肽标签 (CCPGCC) 融合的蛋白的哺乳动物细胞系。只有在加入标记试剂时，标签蛋白才会发荧光。BAL了解更多信息
| 货号 | 数量 |
|---|---|
| T34561 | 1 Kit |
货号 T34561
价格（CNY)
13,588.00
飞享价
Ends: 31-Dec-2025
18,302.00共减 4,714.00 (26%)
1 kit
数量:
1 Kit
价格（CNY)
13,588.00
飞享价
Ends: 31-Dec-2025
18,302.00共减 4,714.00 (26%)
1 kit
TC-FlAsH™ II 细胞内四半胱氨酸标记检测试剂盒包含一种用于活细胞标记的基于表达标记的荧光标记试剂。可使用试剂盒中包含的绿色荧光 FlAsH-EDT2 试剂标记表达与四半胱氨酸短肽标签 (CCPGCC) 融合的蛋白的哺乳动物细胞系。只有在加入标记试剂时，标签蛋白才会发荧光。BAL 洗涤缓冲液替代以前提供的分散式蓝色 3 和基于 EDT 的洗涤缓冲液，作为一种气味更令人满意且产生的信号优于噪声的试剂。
该试剂盒包含 FlAsH-EDT2 标记试剂（在 -20°C 下避光储存）和 BAL 洗涤缓冲液（在 4°C 下储存）。根据指示储存时，试剂盒可稳定保存 6 个月。
该试剂盒包含 FlAsH-EDT2 标记试剂（在 -20°C 下避光储存）和 BAL 洗涤缓冲液（在 4°C 下储存）。根据指示储存时，试剂盒可稳定保存 6 个月。
仅供科研使用。不可用于诊断程序。
规格
检测Tetracysteine 标签检测
颜色绿色
检测方法荧光
适用于（设备）荧光显微镜
标签或染料FlAsH
产品线TC-FlAsH II
产品类型四半胱氨酸标签检测试剂盒
数量1 Kit
运输条件湿冰
产品规格试剂盒
Unit Size1 kit
内容与储存
储存在冰箱（-5 至 -30°C）中并避光。
引用和文献 (64)
引用和文献
Abstract
Resolution of de novo HIV production and trafficking in immature dendritic cells.
Journal:Nat Methods
PubMed ID:18059278
'The challenge in observing de novo virus production in human immunodeficiency virus (HIV)-infected dendritic cells (DCs) is the lack of resolution between cytosolic immature and endocytic mature HIV gag protein. To track HIV production, we developed an infectious HIV construct bearing a diothiol-resistant tetracysteine motif (dTCM) at the C terminus
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling.
Journal:J Virol
PubMed ID:15767407
'Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method to dynamically observe HIV-1 Gag within live cells by adding a tetracysteine tag (C-C-P-G-C-C) to the C terminus of Gag in both
Identification of an intracellular trafficking and assembly pathway for HIV-1 gag.
Journal:Traffic
PubMed ID:16683918
'Retroviral Gag proteins are membrane-bound polyproteins that are necessary and sufficient for virus-like particle (VLP) formation. It is not known how Gag traffics through the cell or how the site of particle production is determined. Here we use two techniques, biarsenical/tetracysteine (TC) labeling and release from a cycloheximide block, to
Real-time visualization of HIV-1 GAG trafficking in infected macrophages.
Journal:PLoS Pathog
PubMed ID:18369466
'HIV-1 particle production is driven by the Gag precursor protein Pr55(Gag). Despite significant progress in defining both the viral and cellular determinants of HIV-1 assembly and release, the trafficking pathway used by Gag to reach its site of assembly in the infected cell remains to be elucidated. The Gag trafficking
Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosis.
Journal:Nat Cell Biol
PubMed ID:17721514
'In addition to their role in cellular bioenergetics, mitochondria also initiate common forms of programmed cell death (apoptosis) through the release of proteins such as cytochrome c from the intermembrane and intracristal spaces. The release of these proteins is studied in populations of cells by western blotting mitochondrial and cytoplasmic