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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, WesternDot™ 585
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Rabbit IgG (H+L) Secondary Antibody (W10804) in WB
Comparison of enhanced chemiluminescence (ECL) and WesternDot® fluorescence-based western blot protein detection. Serial dilutions (750-3 ng protein) of GAPDH were run on NuPAGE® Novex® 4-12% Bis-Tris precast gels (Product # NP0321BOX) and transferred to iBlot® nitrocellulose membranes (Product # IB3010-32) using the iBlot® Gel Transfer Device (Product # IB1001). The membranes were probed with anti-GAPDH an... View More
产品信息
W10804
应用
建议稀释比
免疫印迹 (WB)
1:500
产品规格
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Rabbit Immunoglobins
偶联物
形式
Liquid
保存条件
4° C, do not freeze
RRID
AB_2556786
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
WesternDot fluorescently labeled secondary antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to enhanced chemiluminescence (ECL)-based methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly needed with ECL or colorimetric detection. Differently labeled WesternDot antibodies can be applied to a single blot for simultaneous detection of multiple proteins using standard gel or blot imaging platforms (a list of instruments that are compatible with WesternDot detection can be found in the user manual below). Our WesternDot antibodies utilize Qdot nanocrystals enhanced with VIVID technology, making them brighter than the original Qdot reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
