Interplay between Calcium, Diacylglycerol, and Phosphorylation in the Spatial and Temporal Regulation of PKCalpha -GFP.
Authors: Tanimura Akihiko; Nezu Akihiro; Morita Takao; Hashimoto Noboru; Tojyo Yosuke;
Journal:J Biol Chem
PubMed ID:11997388
The function of protein kinase C (PKC) is closely regulated by its subcellular localization. We expressed PKCalpha fused to green fluorescent protein (PKCalpha-GFP) and examined its translocation in living and permeabilized cells of the human parotid cell line, HSY-EB. ATP induced an oscillatory translocation of PKCalpha-GFP to and from the ... More