This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column. You can expect a purity level of >95% human CD8+ cells.
Advantages:
• No risk of your results being affected by the isolation method • Gentle tube-based method - no columns needed • High purity and recovery • Bead-free cells - compatible with flow cytometry analysis • Compatible with flow cytometry analysis • Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.
The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.
Starting sample: MNC or whole blood.
Capacity: The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeHuman CD8+ T cells
Product LineDynabeads™, FlowComp™
Product TypeHuman CD8 Isolation Kit
Quantity3 mL
Sufficient For2 x 109 cells
CapacityProcesses ∼2 x 109 cells total
Stationary PhasePBMC, Blood
Purity or Quality GradeResearch Grade
Shipping ConditionRoom Temperature
SpeciesHuman
MatrixMagnetic Beads
Unit Size3 mL
Contents & Storage
The Kit Contains: • 1 mL Antibody • 3 mL FlowComp™ Dynabeads™ • 40 mL FlowComp™ Release Buffer.