Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation

Dynabeads Mouse T-Activator CD3/CD28 beads are 4.5-μm superparamagnetic beads covalently coupled with an optimized combination of anti-mouse CD3 and anti-mouse CD28 antibodies and intended for ex vivo activation and expansion/proliferation of mouse T cells. T cells are one of the most important cells in the immune system and are responsible for fighting off infections and cancer and are therefore intensively used in many cancer and disease studies, including CAR-T cell research.

Benefits of Dynabeads Mouse T-Activator CD3/CD28 beads include:
• Easy—activate T cells without the need for feeder cells (antigen-presenting cells)
• Efficient—reproducible method and high T-cell expansion rate (up to 1000-fold)
• No contamination—using magnetic beads avoids contamination of soluble antibodies or mitogens
• Functional­—activated T cells retain the in vivo-like function

Simple and fast procedure
Dynabeads Mouse T-Activator CD3/CD28 beads offer a simple method for the activation and expansion of CD3+ T cells, CD4+ T helper cells or regulatory T cells, and CD8+ T cytotoxic cells isolated from the spleen, lymph nodes, mononuclear cells (MNC’s), or whole blood. Isolated T cells are activated by adding the beads and recombinant mouse or human IL-2 (rIL-2) to the cell culture. Optimal expansion is performed when appropriate T-cell medium is used, such as CTS OpTmizer T Cell Expansion SFM or Advanced RPMI 1640 Medium with added supplements according to the manual.

Activation of mouse T cells
Short-time activation: activation of T cells with Dynabeads Mouse T-Activator CD3/CD28 for a few hours to a few days. This procedure does not expand the cells, and proteomics expression/detection and nucleic acid assays can be performed. The cell:bead complexes cannot be used in flow cytometry in this phase.

Long-term activation/expansion: activation of T cells with Dynabeads Mouse T-Activator CD3/CD28 for three days to a few weeks to obtain an expansion of the T cells. When experiencing reduced expansion or cell shrinking, the T cells can be restimulated with the Dynabeads Mouse T-Activator CD3/CD28 beads. The beads can be removed with a DynaMag magnet and used in applications like cell:cell interaction studies, flow cytometry, proteomics, or nucleic acid-based assays.

Pre-isolate the mouse T cells
To obtain the most optimal activation and expansion, pure T cells can be isolated directly from single cell suspension from mouse spleen or lymph nodes with Dynabeads magnetic beads. For positive isolation of mouse CD3, CD4, or CD8 T cells with a release, use our Dynabeads Mouse FlowComp products. For negative isolation of mouse CD3, CD4, or CD8 T cells, use our Dynabeads Untouched Mouse cell products.

Alternative human activation products, from research to clinic
• For activation of polyclonal T cells, use Dynabeads Human T Activator CD3/CD28
• For activation of regulatory T cells (Tregs), use Dynabeads CD3/CD28 Treg Expander
• For activation of antigen-specific T cells, use Dynabeads Human T Activator CD3/CD28/CD137
• For ex vivo activation and expansion of T cells for cell-based therapy, use the aseptically manufactured CTS Dynabeads CD3/CD28

Commercial supply
Our manufacturing sites are ISO 13485–certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are utilizing Dynabeads Mouse T-Activator CD3/CD28 beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page.