SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
For superior performance, upgrade to the SuperScript IV UniPrime One-Step RT-PCR System
SuperScript&trade; III One-Step RT-PCR System with Platinum&trade; <i>Taq</i> DNA Polymerase
Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection andRead more
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Catalog NumberNo. of Reactions
12574026100 Reactions
1257401825 Reactions
Catalog number 12574026
Price (CNY)/ Each
Price:10,781.00
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No. of Reactions:
100 Reactions
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Price (CNY)/ Each
Price:10,781.00
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SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
Catalog number12574026
Price (CNY)/ Each
Price:10,781.00
-
Add to cart

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 μg of total RNA.

Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
• Sensitive detection down to 0.01 pg total RNA
• Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability
• SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility

SuperScript III Reverse Transcriptase
SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.

Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.

Note: This kit has been optimized for end-point RT-PCR. For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodGel Electrophoresis
FormatOne-Step RT-PCR System
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum Taq DNA Polymerase
Reaction Speed30 min.
Technique1-Step RT-PCR
Optimal Reaction Temperature50°C
Quantity100 rxns
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 4.5 kb
Starting MaterialRNA
Unit SizeEach
Contents & Storage

• SuperScript III RT/Platinum Taq Mix (200 μL)
• 2X Reaction Mix (3 x 1 mL)
• 5 mM Magnesium Sulfate (500 μL)

Store at –10 to –30°C.