TRIzol™ Max™ Bacterial RNA Isolation Kit
TRIzol™ Max™ Bacterial RNA Isolation Kit
Invitrogen™

TRIzol™ Max™ Bacterial RNA Isolation Kit

The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact totalRead more
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Catalog NumberQuantity
16096020100 Preps
16096040200 Preps
Catalog number 16096020
Price (CNY)/ 100 preps
-
Quantity:
100 Preps

The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol™ Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.

Key Features of the TRIzol™ Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps

Two Products in One Kit
The TRIzol™ Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol™ Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol™ Reagent generates a high-quality, minimally degraded RNA product.

Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol™ Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologyOrganic Extraction
Purification Time1 hr.
Elution Volume50 μL
Sample TypeBacteria (Gram +/-)
Final Product TypeTotal RNA
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity100 Preps
Shipping ConditionRoom Temperature
Starting Material AmountCells: ≤108
Bacterial culture: 1.5 mL
YieldGram-negative bacteria: >20 μg
Gram-positive bacteria: ≤3 μg
Unit Size100 preps
Contents & Storage
• 20 mL Max Bacterial Enhancement Reagent; room temperature
• 100 mL TRIzol Reagent; room temperature