DNase I, Amplification Grade

DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. It is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.

Application
Removing DNA from RNA and protein preparations.

Specific activity
Specific activity is >10,000 units/mg.

Source
Purified from bovine pancreas

Performance and quality testing
Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined.

Unit definition
One unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.001 A₂₆₀ units/min/mL of reaction mixture at 25°C.

Unit reaction conditions
0.1 M sodium acetate (pH 5.0), 5 mM MgCl₂, 50 μg/mL calf thymus DNA, and enzyme in 1 mL for 10 min at 25°C.