DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA. Application: Labeling DNA with either radiolabeled or biotinylated nucleotides. Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964. Performance and Quality Testing: Performance tested in nick translation reaction. Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer. Unit Reaction Conditions: 50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/ml template•primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Description
DNA Polymerase
Polymerase
DNA Polymerase I
Exonuclease Activity
3' - 5', 5' - 3'
Quantity
250 U
Shipping Condition
Wet Ice
Hot Start
No
Unit Size
250 units
Contents & Storage
DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.