EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of the EGS crosslinker:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

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EGS (ethylene glycol bis(succinimidyl succinate))