Gibco™

MEM, HEPES, no glutamine

Catalog number: 32360034
Gibco™

MEM, HEPES, no glutamine

Catalog number: 32360034
Catalog Number
32360034
Unit Size
10 x 500 mL
Price (CNY)
Catalog NumberUnit SizePrice (CNY)
3236003410 x 500 mLContact Us ›
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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM is modified as follows:
WithWithout
• HEPES• L-glutamine
• Phenol Red 


The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Culture Environment
CO2
Cell Type
Mammalian Cells
Classification
Animal Origin-free
Concentration
1 X
Culture Type
Mammalian Cell Culture
Form
Liquid
Serum Level
Standard Serum Supplementation
Shelf Life
12 Months
Sterility
Sterile-filtered
With Additives
HEPES, Phenol Red, Sodium Bicarbonate
Without Additives
No Glutamine, No Sodium Pyruvate
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Osmolality
280 - 320 mOsm/kg
pH
7 to 7.4
Product Line
Gibco™
Quantity
10 x 500 mL

Contents & Storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

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