Invitrogen

Metadherin Polyclonal Antibody

Metadherin Antibody in Immunocytochemistry (ICC/IF)

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Metadherin Antibody (40-6400) in ICC/IF

Immunofluorescence analysis of Metadherin was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Metadherin Rabbit Polyclonal Antibody (Product # 40-6400) at 1.5 µg/mL and incubated for 3 hours at room temperature an... View More

Metadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Metadherin Antibody in Western Blot (WB)

Metadherin Antibody in Immunohistochemistry (IHC)

Metadherin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Metadherin Antibody in Immunoprecipitation (IP)

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产品信息

40-6400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1-2 µg/mL

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Raised against the C-term of human Metadherin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533474

靶标信息

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.

Pathology international

Lin LW,Lai PS,Chen YY,Chen CY

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Mon Nov 01 00:00:00 UTC 2021

Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.

Pathology international

Lin LW,Lai PS,Chen YY,Chen CY

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Mon Nov 01 00:00:00 UTC 2021

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Western Blot

Tue Sep 29 00:00:00 UTC 2020

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Western Blot

Tue Sep 29 00:00:00 UTC 2020

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Western Blot

Tue Sep 29 00:00:00 UTC 2020

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Western Blot

Tue Sep 29 00:00:00 UTC 2020

Astrocyte-elevated gene-1 confers resistance to pemetrexed in non-small cell lung cancer by upregulating thymidylate synthase expression.

Oncotarget

Chen CY,Chen YY,Chen JJW,Chen KY,Ho CC,Shih JY,Chang YL,Yu CJ,Yang PC

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Fri Sep 22 00:00:00 UTC 2017

MiR-630 suppresses breast cancer progression by targeting metadherin.

Oncotarget

Zhou CX,Wang CL,Yu AL,Wang QY,Zhan MN,Tang J,Gong XF,Yin QQ,He M,He JR,Chen GQ,Zhao Q

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Western Blot

Tue Jan 12 00:00:00 UTC 2016

MiR-630 suppresses breast cancer progression by targeting metadherin.

Oncotarget

Zhou CX,Wang CL,Yu AL,Wang QY,Zhan MN,Tang J,Gong XF,Yin QQ,He M,He JR,Chen GQ,Zhao Q

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Western Blot

Tue Jan 12 00:00:00 UTC 2016

Astrocyte elevated gene-1 interacts with β-catenin and increases migration and invasion of colorectal carcinoma.

Molecular carcinogenesis

Zhang F,Yang Q,Meng F,Shi H,Li H,Liang Y,Han A

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Thu Aug 01 00:00:00 UTC 2013

Astrocyte elevated gene-1 interacts with β-catenin and increases migration and invasion of colorectal carcinoma.

Molecular carcinogenesis

Zhang F,Yang Q,Meng F,Shi H,Li H,Liang Y,Han A

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Thu Aug 01 00:00:00 UTC 2013

Astrocyte elevated gene-1 interacts with β-catenin and increases migration and invasion of colorectal carcinoma.

Molecular carcinogenesis

Zhang F,Yang Q,Meng F,Shi H,Li H,Liang Y,Han A

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Thu Aug 01 00:00:00 UTC 2013

Pathologically decreased miR-26a antagonizes apoptosis and facilitates carcinogenesis by targeting MTDH and EZH2 in breast cancer.

Carcinogenesis

Zhang B,Liu XX,He JR,Zhou CX,Guo M,He M,Li MF,Chen GQ,Zhao Q

Sat Jan 01 00:00:00 UTC 2011

生物信息学

蛋白别名: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC

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基因别名: 2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; AEG1; AV353288; D8Bwg1112e; LYRIC; LYRIC/3D3; MTDH

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UniProt ID: (Human) Q86UE4, (Mouse) Q80WJ7

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Entrez Gene ID: (Human) 92140, (Mouse) 67154

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功能

RNA polymerase II transcription factor binding transcription coactivator activity double-stranded RNA binding protein binding poly(A) RNA binding NF-kappaB binding

参与通路

negative regulation of transcription from RNA polymerase II promoter positive regulation of autophagy lipopolysaccharide-mediated signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling bicellular tight junction assembly