High-Capacity RNA-to-cDNA™ Kit
High-Capacity RNA-to-cDNA™ Kit
Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression MasterRead more
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Catalog NumberNo. of Reactions
438740650 Reactions
4388950500 Reactions
Catalog number 4387406
Price (CNY)/ 50 reactions
Price:3,932.00
Online offer:2,360.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Your Price:
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Add to cart
No. of Reactions:
50 Reactions
Price (CNY)/ 50 reactions
Online offer:2,360.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Add to cart
High-Capacity RNA-to-cDNA™ Kit
Catalog number4387406
Price (CNY)/ 50 reactions
Online offer:2,360.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Add to cart

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Reaction Speed30 to 60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature37°C
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 7 kb
Starting MaterialRNA
Unit Size50 reactions
Contents & Storage

• 20X Enzyme mix (1 x 50 μL)
• 2X RT Buffer Mix (1 x 500 μL)

Store at –15 to –25°C.
Buffer Mix may be stored at 2–8°C for up to 6 months.