Silencer™ Select Negative Control No. 1 siRNA

The Silencer™ Select Negative Control No. 1 siRNA is a non-targeting negative control siRNA with the same chemical modifications for enhanced efficacy as in other Silencer™ Select siRNAs. Properties of Silencer™ Select control siRNAs:

• Validated siRNA controls for optimizing siRNA experiments
• GAPDH Positive Control functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer™ Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer™ Select siRNAs?
Silencer™Select siRNAs are our next-generation siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer™ Select Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. We have designed and extensively tested two Silencer™ Select Negative Control siRNAs. These siRNAs include the same modifications for reducing off-target effects as found in other Silencer™ Select siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these two controls have been tested in multi-parametric, cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality control
We synthesize and purify each Silencer™ Select siRNA in state-of-the-art facilities to meet the highest-quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.