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As an integral component of the Personal Genome Machine™ sequencing platform, the Ion Plus Fragment Library Kit is designed to produce high quality DNA libraries via multiple possible workflows. The economically priced Ion Plus Fragment Library Kit provides low cost sample preparation, bringing next generation sequencing to every lab. This revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries when combined with either physical shearing methods or our proprietary Ion Shear™ enzymatic fragmentation technology (Ion Xpress Plus Library Kit, Cat. No. 4471269), thus saving time and cost. With significantly higher yields than other library construction techniques, high quality libraries can be attained with as little as 100 ng input DNA. In addition, this highly efficient library construction process enables the creation of “amplification-free” libraries from <1 ug input material. This kit is also extremely flexible in that it is compatible with multiple physical shearing and library size selection methods.
The Ion Plus Fragment Library Kit contains sample preparation reagents for library construction of up to 20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. Store components at -20°C.
The Ion Plus Fragment Library Kit offers you:
• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes with a choice of mix-and-match workflows
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System
A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With a quick turnaround time the Ion Plus Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your samples possible in less time than ever before. Furthermore, the Ion Plus Fragment Library Kit is compatible with a variety of DNA shearing and library size-selection methodologies allowing you to tailor your workflow to suit your project needs.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
The Ion Plus Fragment Library Kit contains sample preparation reagents for library construction of up to 20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. Store components at -20°C.
The Ion Plus Fragment Library Kit offers you:
• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes with a choice of mix-and-match workflows
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System
A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With a quick turnaround time the Ion Plus Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your samples possible in less time than ever before. Furthermore, the Ion Plus Fragment Library Kit is compatible with a variety of DNA shearing and library size-selection methodologies allowing you to tailor your workflow to suit your project needs.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Libraries
Fragment Library
For Use With (Equipment)
Ion PGM™ System, Ion OneTouch™ System
High-throughput Compatibility
High-throughput Compatible, Multiplexing
Sample Type
DNA
Sequencing Type
Genome & DNA Sequencing
Shipping Condition
Dry Ice
Workflow Step
Library Generation
Product Line
Ion Torrent™, Ion Xpress™
For Use With (Application)
Post-Light™ Ion Semiconductor Sequencing
Contents & Storage
5× End Repair Buffer,1 tube, 400 μL,–°C to –10°C
End Repair Enzyme,1 tube, 20 μL,–°C to –10°C
10× Ligase Buffer,1 tube, 200 μL, –°C to –10°C
DNA Ligase, 1 tube, 40 μL, –°C to –10°C
Adapters, 1 tube, 100 μL, –°C to –10°C
Platinum™ PCR SuperMix High Fidelity, 2 tubes, 1 mL, –°C to –10°C
Library Amplification Primer Mix,1 tube, 100 μL, –°C to –10°C
Nick Repair Polymerase,1 tube, 160 μL, –°C to –10°C
dNTP Mix, 1 tube, 40 μL, –°C to –10°C
Low TE, 2 tubes, 1.5 mL 15 to °C or
–°C to –10°C
End Repair Enzyme,1 tube, 20 μL,–°C to –10°C
10× Ligase Buffer,1 tube, 200 μL, –°C to –10°C
DNA Ligase, 1 tube, 40 μL, –°C to –10°C
Adapters, 1 tube, 100 μL, –°C to –10°C
Platinum™ PCR SuperMix High Fidelity, 2 tubes, 1 mL, –°C to –10°C
Library Amplification Primer Mix,1 tube, 100 μL, –°C to –10°C
Nick Repair Polymerase,1 tube, 160 μL, –°C to –10°C
dNTP Mix, 1 tube, 40 μL, –°C to –10°C
Low TE, 2 tubes, 1.5 mL 15 to °C or
–°C to –10°C
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