The ViewRNA Cell Plus Assay is a novel assay that combines immunocytochemistry with ViewRNA technology, a proprietary fluorescent in situ hybridization and sequential branched-DNA amplification technique, to visualize both RNA with single-molecule sensitivity and protein in individual cells. This assay enables simultaneous detection of up to three RNA targets in combination with immunophenotyping for cell surface or intracellular proteins using both indirect and direct immunocytochemistry to allow for detailed characterization of specific cell subpopulations. For simultaneous detection of a fourth RNA target, the ViewRNA ISH Cell 740 Module (Cat. No. QVC0200) can be combined with this kit. It allows analysis of an addtional target in the 740 channel (AlexaFluor 750).

This ViewRNA Cell Plus Assay kit contains all the reagents needed to conduct the assay. Target Probe sets for genes of interest and antibodies are sold separately.

Reactivity/species
Probes can be designed for any species. These are the species most frequently used/tested: Armenian hamster, baboon, bovine, canine, cat, chicken, chimpanzee, cow, cynomolgus monkey, donkey, goat, golden Syrian hamster, guinea pig, hamster, horse, human, macaque, monkey, mouse, non-human primate, olive baboon, pig, pigtailed macaque, rabbit, rat, rhesus monkey, sheep

Reported application
Microscopy, immunocytochemistry