The Dynabeads MAX EPEC/VTEC O121 Kit allows for the rapid, selective concentration of E. coli O121 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the Thermo Scientific KingFisher mL Food Protection Purification System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Sensitivity
Following the Dynabeads MAX EPEC/VTEC O121 protocol, the presence or absence of one viable E. coli O121 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

NOTE: Lot 1375851 of this product contains only 1 vial of paramagnetic beads (1 mL each) sufficient for 100 reactions. This batch follows a slightly modified protocol (see use instructions in document part number MAN0007304 Revision 1.0).