Invitrogen™

RiboMinus™ Eukaryote System v2

Catalog number: A15026
Invitrogen™

RiboMinus™ Eukaryote System v2

Catalog number: A15026
Catalog Number
A15026
Unit Size
12 reactions
Price (CNY)
Price: 17,283.00
Online offer: 14,690.00
(ends 31-Dec-2024)
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (CNY)AvailabilityQuantity
A1502612 reactions
Price: 17,283.00
Online offer: 14,690.00
(ends 31-Dec-2024)
Your price:
-
Product Overview
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The RiboMinus™ Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the cytoplasmic (5S, 5.8S, 18S, and 28S) and mitochondrial (12S and 16S) ribosomal RNA from total RNA. This selection system contrasts with poly-A and cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the RiboMinus™ Eukaryote System v2 include:

• Magnetic bead-based purification for rapid, seamless purification
• A 1-hour workflow
• An input range of 1–5 μg

The RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote System v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote System v2 is designed for the removal of human, mouse, and rat rRNA. A magnetic bead-based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non-coding RNA. For column-based purification, please see the RiboMinus™ Eukaryote Kit v2. The RiboMinus Eukaryote System v2 has an RNA input range of 1–5 μg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species
Human, Mouse, Rat
Final Product Type
Transcriptome RNA
For Use With (Equipment)
Ion PGM™ System, Ion Proton™ System, Illumina™ Genome Analyzer II (GA II)
No. of Reactions
12
Product Line
RiboMinus™
Sequencing Type
Transcriptome Sequencing
Starting Material Amount
1 - 5 μg total RNA
Workflow Step
Sample Enrichment

Contents & Storage

• RiboMinus™ Eukaryote Probe Mix v2, store at -20°C
• RiboMinus™ Magnetic Beads, store at 4°C
• 2X Hybridization Buffer, store at room temperature
• Binding Solution Concentrate, store at room temperature
• Wash Solution Concentrate, store at room temperature
• Nucleic Acid Binding Beads, store at 4°C

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