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Invitrogen
Fluorescein/Oregon Green Polyclonal Antibody, HRP
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Application
FIGURE: 1 / 3
Fluorescein/Oregon Green Antibody (A-21253) in ICC/IF
Nuclear and nonnuclear incorporation of 5-bromo-2'-deoxyuridine in live cells. Bovine pulmonary arterial endothelial (BPAE) cells were labeled with 5-bromo-2'-deoxyuridine (BrdU, Product # B23151) applied at a concentration of 10 µM for 30 minutes. After fixation with 4% formaldehyde in phosphate-buffered saline for 30 minutes, chromatin was denatured by treatment with 2 M HCl for 20 minutes. Incorporated B... View More
Product Details
A-21253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fluorescein/Oregon Green
Conjugate
HRP
Form
Lyophilized
Purification
IgG fraction
Storage buffer
0.1M potassium phosphate, pH 8
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.
Store product at -20°C until opened. Reconstitute using 0.5 mL PBS, pH 7.2, to yield a 1 mg/mL stock solution. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.
Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.
Target Information
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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