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Alexa Fluor 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor 647 dye.
• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 650/668 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor 647 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor 647 phalloidin allows high-contrast discrimination of actin staining.
Use in Multiple Applications
Alexa Fluor 647 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor 647 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.
See User Manual for solubility instructions.
Related Links
• Read about labeling F-Actin with phallotoxins.
• See the full line of Alexa Fluor Dye products.
• Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool.