RapidFinder™ Beef ID Kit

The RapidFinder™ Beef ID Kit enables the simple and reliable detection of beef in food and feed samples. The method works by detection of bovine, or Bos taurus, DNA by real-time PCR. Unlike ELISA-based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Beef ID Kit detects both raw and processed beef and provides sensitivity down to 0.01% bovine DNA. The included positive control, which comprises 0.1% bovine DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-bovine DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process-control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Beef ID Kit works
Bovine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Bos taurus. The target sequence, detected in the FAM™ dye channel, is not only specific for beef, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% bovine DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is optimized and validated for use with the Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR Instrument and the Applied Biosystems™ 7500 Fast Real‑Time PCR Instrument.

Sample preparation
DNA sample preparation for the RapidFinder™ Beef ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Beef ID Kit, most labs will go on to quantify the amount of bovine DNA in the sample. This can be accomplished using the RapidFinder™ Beef ID Kit in conjunction with the RapidFinder™ Quant Multi-Animal Set (Cat. No. A24399).

Part of a suite of solutions for animal species detection
The RapidFinder™ Beef ID Kit is just one of our many kits available for the sensitive detection of various animal species in food and feed samples. Other RapidFinder™ animal species identification kits available include those for the detection of goat, rabbit, water buffalo, duck, pork, horse, sheep, ruminant, chicken, turkey, and poultry.

The RapidFinder™ Animal Species Identification kits are made available through a partnership with Health in Code, S.L.