Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Thermo Scientific Pierce DTT (Dithiothreitol), No-Weigh format, is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M single-use stocks for addition to protein samples for effective reduction of disulfide bonds.

DTT is a popular reducing agent for many protein research applications, including preparing proteins for reducing polyacrylamide electrophoresis (SDS-PAGE) and reducing disulfide bonds for labeling sulfhydryl groups in conjugation reactions. At low concentrations, DTT is used to stabilize proteins and enzymes with free sulfhydryl groups to keep monothiols in a reduced state. DTT reduction is also used to prepare proteins for mass spectrometry.

Properties of DTT:

• Synonyms—1,4-dithiothreitol; DTT; Cleland’s Reagent; 1,4-dimercapto-2,3-butanediol
• Molecular weight (MW)—154.24
• Molecular formula—C₄H₁₀O₂S₂
• CAS number—3483-12-3

DTT is not stable in solution and should be prepared fresh for each use. The No-Weigh format consists of a 48-microtube tray containing dry, stable, single-use aliquots of the DTT chemical to eliminate the need to weigh small amounts of reagent and prevent waste of unused DTT. Just puncture the seal of one microtube, add 100 μL of water or buffer, and mix to dissolve. In just a few seconds you will have a solution of 500 mM DTT, ready for a variety of laboratory methods. Dilute as necessary or add the appropriate volume of the 500 mM stock solution to achieve the amount of DTT reducing agent required for your specific application.

Features of the No-Weigh format:

• Saves time—just add 100 μL water or buffer to make 500 mM DTT solution in seconds
• Eliminates waste—makes 100 μL at a time, just the right amount for most applications
• Always fresh—ensures full reducing strength for every use; no need to store stock solutions

With the convenient No-Weigh format, a 500 mM DTT solution can be made in less than 30 seconds. The unique packaging ensures that the DTT reducing agent is at full strength and able to protect proteins from oxidative damage or reduce any disulfides prior to electrophoresis.