Pierce™ Alkylating Reagents

Thermo Scientific Pierce Iodoacetamide or Chloroacetamide are provided in a no-weigh format, where small amounts of reagent are pre-weighed and packaged for single use. This single-use format prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. The format enables use of a fresh vial of reagent each time, and eliminates the hassle of weighing out small amounts of reagent.

Alkylating reagents iodoacetamide and chloracetamide are sulfhydryl-reactive alkylating reagents used to block reduced cysteine residues for protein characterization and peptide mapping. Alkylation with iodoacetamide or chloracetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. Reducing agents added after alkylation will react with excess iodoacetamide.