The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.
Features of the AAV-MAX Helper-Free AAV Production System include:
• Components work synergistically to produce high AAV titers greater than 5 x 10
10 vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection
The components included in the AAV-MAX System Kit are:
• 1 vial of
Viral Production Cells 2.0 (CN) (locally manufactured in China) • 1 L of
Viral Production Medium • 1
AAV-MAX Transfection Kit for 1 L of production
• 100 mL
Viral-Plex Complexation Buffer • 100 mL
AAV-MAX Lysis Buffer All components are also available for purchase separately. Note that this kit includes Viral Production Cells 2.0 (CN), which are locally manufactured in China. For the kit that contains Viral Production Cells 2.0, please see
Cat. No. A51217.