SureTect ™ Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Assay

Rapidly detect Vibrio cholerae, V. parahaemolyticus and V. vulnificus in food samples in as little as 10 hours with highly specific, simple-to-use real-time PCR Thermo Scientific™ SureTect Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Assay.

The SureTect assay format incorporates color-coded plastics with orientation markings and breakable strip format, colored reagents, and pierceable seals to enable an optimized workflow for maximum laboratory efficiency and productivity.

The assay has been AOAC-RI validated for fish and seafood products with a wide range of test portion sizes.

The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.

Simple

• Easy-to-use with single enrichment step and pre-dispensed reagents
• Target-specific colored assay plastics
• Clearly oriented lysis and assay trays
• Color indicator in lysis reagent reduces the possibility for human error

• Option to automate the lysis and PCR sample preparation
• Optimized workflow delivers on-screen results in as little as 10 hours
• Maximum flexibility for operational efficiency
• Run all assays simultaneously in the same run
• Test up to 96 samples in each run
• Choice of pierceable seals or caps
• Detect multiple targets in the same run with universal PCR protocols across the range

 Smart

• Intuitive, user-friendly software delivers reliable results, with no subjective interpretation
• Actionable data supports fast decisions in a complex product supply chain
• IPC in every assay for complete confidence in results

The Thermo Scientific™ Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Assay includes.

• Lysis Reagent 1 Tubes
• Proteinase K
• Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Tubes
• ThermoScientific 96-Well Pierceable Seals
• PCR caps

Enriched samples are pipetted into pre-filled lysis tubes, along with Proteinase K, before incubation to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Vibrio cholerae, V. parahaemolyticus and V. vulnificus PCR tube to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers, and DNA template for the internal positive control (IPC).

The PCR tubes are sealed, then loaded into the Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR instrument using RapidFinder™ Analysis Software v3.0 (or later) or the Applied Biosystems 7500 Fast Food Safety 96-well Real-Time PCR instrument using RapidFinder™ Express Software v2.0 (or later). After completing the run, interpreted results are clearly displayed and can be reported, stored, printed, or downloaded as required. The internal positive control confirms correct assay conditions have occurred.