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Invitrogen
TRITC Polyclonal Antibody
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FIGURE: 1 / 4
TRITC Antibody (A-6397) in IHC
BMC genomics 2019 -
Fig. 1 Mouse pyramidotomy model in neonates and adults. a - d , Representative images of immunohistochemistry for PKCgamma (a corticospinal tract (CST) marker) using the cervical cord of mice 4 weeks after injury. The dorsal column of sham ( a , c ) or pyramidotomy ( b , d ) groups, which were injured at P7 ( a , b ) or 8 weeks of age ( c , d ), are shown. White broken lines indicate the denervated side of ... View More
Product Details
A-6397
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Tetramethylrhodamine
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence. These anti-dye antibodies, including those that recognize the tetramethylrhodamine (TRITC) and Texas Red™ fluorophores, can also serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes. Our anti-tetramethylrhodamine (A6397) and anti-Texas Red (A6399) antibodies have been raised against the tetramethylrhodamine and Texas Red fluorophores, respectively. These antibodies quench much of the fluorescence of the complementary dyes. However, due to the related chemical structures, the anti-tetramethylrhodamine antibody crossreacts with the Texas Red fluorophore and the anti-Texas Red antibody cross-reacts with tetramethylrhodamine. Also, both antibodies tightly bind the Rhodamine Red™ fluorophore. Thus, either antibody can serve as an effective probe for tetramethylrhodamine, Texas Red, or Rhodamine Red dyes.
We use a sensitive quenching assay to ensure that this antibody is provided at a consistently high titer value. As supplied, 20 µL of the antibody solution is certified to produce ≥50% of the maximal fluorescence quenching of 1 mL of a 50 nM solution of tetramethylrhodamine, assayed in 100 mM sodium phosphate, pH 8.0. Maximal quenching for tetramethylrhodamine is ~80% of the fluorescence of the free dye. Due to steric hindrance, maximal fluorescence quenching of the tetramethylrhodamine covalently bound to protein may be significantly less.
Target Information
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent traditional dye with excitation ideally suited to the 532 nm laser line. It has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence and other cellular imaging applications. Unlike biotin, which is an endogenous ligand in mitochondria, dye-based haptens permit background-free staining of cells and tissues and a sensitive alternative to biotin-streptavidin methods.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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