CellTracker™ Fluorescent Probes
CellTracker™ Fluorescent Probes
Invitrogen™

CellTracker™ Fluorescent Probes

CellTracker™ Probes are nontoxic, fluorescent dyes well suited for monitoring cell movement or location. After loading into cells, the dyes are well retained allowing for multigenerational tracking of cellular movements.
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Catalog NumberDescriptionQuantity
A66462CellTracker™ Red CMTPX5 x 50 μg
A66433CellTracker™ Deep Red15 μg
A66435CellTracker™ Red CMTPX50 μg
A66432CellTracker™ CM-DiI50 μg
A66434CellTracker™ Green CMFDA50 μg
A66459CellTracker™ CM-DiI S5 x 50 μg
A66460CellTracker™ Deep Red5 x 15 μg
A66461CellTracker™ Green CMFDA5 x 50 μg
Catalog number A66462
Price (CNY)/ Each
Price:2,224.00
Online offer:1,668.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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Description:
CellTracker™ Red CMTPX
Quantity:
5 x 50 μg
Price (CNY)/ Each
Online offer:1,668.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Add to cart
CellTracker™ Fluorescent Probes
Catalog numberA66462
Price (CNY)/ Each
Online offer:1,668.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Add to cart
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. CellTracker Fluorescent Probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

Features of the CellTracker dyes:
• Easy to use—remove culture media, add dye, incubate 15-45 minutes, and image cells
• Excellent retention—fluorescent signal retention of >72 hours (typically three to six generations)
• Ideal tracking dyes—monitor cell movement, location, proliferation, migration, chemotaxis, and invasion
• Low cytotoxicity—does not affect viability or proliferation
• Cell versatility–works with cells in suspension and adherent cells

The CellTracker fluorescent probes have been designed to freely pass through cell membranes; however, once inside the cell are transformed into cell-impermeant reaction products. The CellTracker Green and Red fluorescence probes contain a chloromethyl or bromomethyl group that reacts with thiol groups, utilizing a glutathione S-transferase–mediated reaction. In most cells, glutathione levels are high (up to 10 mM) and glutathione transferase is ubiquitous. The CellTracker Deep Red probe contains a succinimidyl ester reactive group, which reacts with amine groups present on proteins.

The strong, photostable fluorescence, excellent cellular retention, and minimal cytotoxicity of DiI and other long-chain carbocyanine membrane probes make them particularly suitable for long-term labeling and tracking of cells, intracellular membranes, liposomes, viruses, and lipoproteins. CellTracker CM-DiI incorporates a mildly thiol-reactive chloromethyl substituent that confers aldehyde fixability via conjugation to thiol-containing peptides and proteins. Unlike DiI and PKH26, CM-DiI is retained in cells throughout fixation, permeabilization, and paraffin embedding procedures.

After conversion to impermeant versions, the CellTracker fluorescent probes are well retained in living cells through several generations. The probes are transferred to daughter cells but are not transferred to adjacent cells in a population. Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, several CellTracker fluorescent probes with various excitation and emission spectra are available allowing for multiplexing in fluorescence imaging. The CellTracker dyes are retained with fixation and permeabilization, enabling their use with antibodies for fluorescence immunostaining applications. In addition to bright fluorescence and excellent retention, the CellTracker fluorescent probes do not contribute to cytotoxicity.

Spectral characteristics of the fluorescent CellTracker probes:
• CellTracker Green CMFDA—492/517 nm
• CellTracker Red CMTPX—577/602 nm
• CellTracker Deep Red—630/660 nm
• CellTracker CM-DiI Dye—553/570 nm

The fluorescent CellTracker reagents include the green-fluorescent chloromethyl derivatives of fluorescein diacetate (CMFDA), red-fluorescent CMTPX and CM-DiI, and Deep Red. CellTracker Red CMTPX does not require enzymatic cleavage to activate its fluorescence, whereas the green CMFDA does require enzymatic cleavage. CMFDA is colorless and non-fluorescent until cytosolic esterases cleave off the acetates, releasing a brightly fluorescent product.

For Research Use Only.
Specifications
ColorRed
Excitation Wavelength Range577/602 nm
For Use With (Application)Cellular Imaging, Cell Analysis, Cellular Imaging
For Use With (Equipment)Fluorescence Microscope
Quantity5 x 50 μg
Detection MethodFluorescence
DescriptionCellTracker™ Red CMTPX
FormDry Powder
Product LineCellTracker™
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationCytoplasm
Molecular Weight (g/mol)698.3
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C), and protect from light.