Gibco™ Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, where they were derived from the pupal ovarian tissue of the fall army worm,
Spodoptera frugiperda. Gibco™ Sf21 cells (frozen in Grace's Insect Medium) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing
Recombinant protein expression from a variety of expression systemsHigh levels of protein expression in Sf21 cells can be obtained using either the
BaculoDirect™ Baculovirus Expression System, the
Bac-to-Bac™ Baculovirus Expression System, or the
InsectDirect™ Expression System.
Good growth in adherent or suspension cultureProtocols for adherent or suspension growth in Gibco™
Supplemented Grace's Insect Medium are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow.
Quality and performance testingEach lot of Gibco™ Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.
Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.