CellLight™ Histone 2B-GFP, BacMam 2.0
CellLight™ Histone 2B-GFP, BacMam 2.0
Invitrogen™

CellLight™ Histone 2B-GFP, BacMam 2.0

CellLight Histone 2B-GFP, BacMam 2.0, provides an easy way to label histone 2b with green fluorescent protein (GFP) in liveRead more
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Catalog number C10594
Price (CNY)/ Each
Price:8,023.00
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Price (CNY)/ Each
Price:8,023.00
Add to cart
CellLight™ Histone 2B-GFP, BacMam 2.0
Catalog numberC10594
Price (CNY)/ Each
Price:8,023.00
-
Add to cart
CellLight Histone 2B-GFP, BacMam 2.0, provides an easy way to label histone 2b with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to histone 2B. You can observe histone 2B-GFP behavior in live cells using fluorescent imaging with almost no cytotoxicity.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight Technology is:
Fast and convenient: simply add CellLight reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight Histone 2B-GFP, BacMam 2.0, is a fusion construct of histone 2B and emGFP, providing accurate and specific targeting to cellular histone 2B-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Excitation Wavelength Range488⁄510
Dye TypeGFP (EmGFP)
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope
Quantity1 mL
Detection MethodFluorescence
EmissionVisible
FormLiquid
Product LineCellLight™
Shipping ConditionWet Ice
TechniqueFluorescence Intensity
Label TypeFluorescent Dye
Product TypeHistone Label
SubCellular LocalizationNucleus
Unit SizeEach
Contents & Storage
Store at 2°C to 6°C, protected from light. Do Not Freeze.