Slanetz and Bartley Medium (Dehydrated)

Detect and enumerate enterococci from water and food samples with Thermo Scientific™ Slanetz and Bartley Medium (Dehydrated). Slanetz and Bartley¹ originally devised this medium to detect and enumerate enterococci by the technique of membrane filtration, but it has also proved useful as a direct plating medium^{2, 3}. The medium is very selective for enterococci and, when it is incubated at elevated temperatures (44-45°C), all red or maroon colonies may be accepted as presumptive enterococci^{4, 5}.

The detection and enumeration of enterococci is of primary importance for food hygiene monitoring and microbial quality of water and food.

The Environment Agency ‘Microbiology of Drinking Water 2002’⁶ recommend the use of Slanetz and Bartley medium for the enumeration of enterococci in water supplies, as does ISO in the standard for water quality⁷. Food samples can be examined for enterococci by the method suggested by the Nordic Committee of Food Analysis³.

• Easy-to-read: due to the reduction of tetrazolium chloride in the medium the enterococci form dark red/ maroon colonies.
• Selective: sodium azide in the medium is selective against Gram-negative organisms.

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