Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn)

Muller-Kauffmann Tetrathionate-Novobiocin Broth was developed by Muller¹ and later modified by Kauffmann²³⁴ with the addition of ox bile and brilliant green to improve selectivity. The addition of novobiocin at 40mg per liter was later described by Jeffries⁵ to improve inhibition of Proteus species. A selective enrichment medium for the isolation of Salmonella, the formulation conforms to ISO 6579:20025. Presumptive Salmonella should be confirmed using appropriate biochemical and serological techniques. For the complete method, please refer to ISO 6579:20021.

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