Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 594 conjugate
<i>Escherichia coli</i> (K-12 strain) BioParticles&trade;, Alexa Fluor&trade; 594 conjugate
Invitrogen™

Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 594 conjugate

The Molecular Probes™ BioParticles™ product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeastRead more
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Catalog number E23370
Price (CNY)/ 2 mg
Price:3,713.00
Online offer:2,859.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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Add to cart
Price (CNY)/ 2 mg
Online offer:2,859.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Add to cart
Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 594 conjugate
Catalog numberE23370
Price (CNY)/ 2 mg
Online offer:2,859.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Add to cart
The Molecular Probes™ BioParticles™ product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles™ products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.

We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles™ conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles™ conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor™, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.

BioParticles Specifications:
• Label (Ex/Em): Alexa Fluor™ 594 (∼590/617 nm)
• Particle: E. coli (K-12 strain)
Opsonizing reagent available


Using BioParticles Products
BioParticles™ conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles™ conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles™ conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles™ conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.

Find More BioParticles™ Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles™ products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook.

For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles™ conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product TypeE.coli Conjugate
Dye TypeAlexa Fluor™ 594
FormLyophilized Powder
pH3 to 10
Product LineAlexa Fluor™, BioParticles™
Quantity2 mg
Shipping ConditionRoom Temperature
SpeciesE. coli
Unit Size2 mg
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.