Exonuclease I (20 U/μL)
Exonuclease I (20 U/μL)
Thermo Scientific™

Exonuclease I (20 U/μL)

Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner  Read more
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Catalog NumberQuantity
EN058220,000 U
EN05814000 U
Catalog number EN0582
Price (CNY)/ 20,000 units
Price:3,784.00
Online offer:2,649.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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Quantity:
20,000 U
Request bulk or custom format
Price (CNY)/ 20,000 units
Online offer:2,649.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Add to cart
Exonuclease I (20 U/μL)
Catalog numberEN0582
Price (CNY)/ 20,000 units
Online offer:2,649.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Add to cart
Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

• Active in PCR buffers

Applications

• Primer removal from PCR mixtures:
• prior to PCR product sequencing
• for one-tube 'megaprimer' PCR mutagenesis

• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration20 U/μL
EnzymeExonuclease
Quantity20,000 U
Product TypeExonuclease I
Unit Size20,000 units