RNase I (10 U/μL)
RNase I (10 U/μL)
Thermo Scientific™

RNase I (10 U/μL)

Thermo Scientific Ribonuclease I (RNase I) is an endoribonuclease that preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2',Read more
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Catalog NumberQuantity
EN06011,000 units
EN06025,000 units
Catalog number EN0601
Price (CNY)/ Each
Price:1,106.00
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Quantity:
1,000 units
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Price (CNY)/ Each
Price:1,106.00
Add to cart
RNase I (10 U/μL)
Catalog numberEN0601
Price (CNY)/ Each
Price:1,106.00
-
Add to cart
Thermo Scientific Ribonuclease I (RNase I) is an endoribonuclease that preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates.

The enzyme does not require any metal ions for activity.

Highlights

• Stable and active under a wide variety of reaction conditions
• Can be heat inactivated in 30 minutes at 100°C

Applications

• Removal of RNA from DNA solutions
• Removal of RNA from recombinant protein preparations
• Ribonuclease protection assays

Notes

Mammalian ribonuclease inhibitors have no effect on RNase I. RNase I binds to DNA, but does not degrade it. RNase I amino acid sequence is typical for the RNase T2 family. Non-ionic detergents (e.g., Triton X-100) do not inhibit RNase I, and may even slightly stimulate its activity and stabilize it against heat inactivation. Triton X-100 or BSA (at 0.1 mg/mL) may prevent RNase I from sticking to glass vessels when working with dilute solutions. Polyamines stimulate the activity of RNase I.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 U/μL
EnzymeRNase
Quantity1,000 units
Product TypeRNase I
Unit SizeEach