DreamTaq DNA Polymerase (5 U/μL)

DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Features

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Incorporates modified nucleotides, but does not incorporate dUTP
• Direct loading of PCR product on gel with green format

Applications

• Routine PCR amplification of DNA fragments up to 6 kb
• High throughput PCR
• Genotyping

Notes

• For applications requiring PCR product analysis by absorbance or fluorescence excitation, colorless 10X DreamTaq Buffer (Cat. No. B65) is recommended for purifying PCR products before analysis.