Lyo-ready SuperScript™ III Flash Reverse Transcriptase (60 U/μL)
Lyo-ready SuperScript™ III Flash Reverse Transcriptase (60 U/μL)
Invitrogen™

Lyo-ready SuperScript™ III Flash Reverse Transcriptase (60 U/μL)

Invitrogen Lyo-ready SuperScript III Flash Reverse Transcriptase joins the SuperScript product family as a reverse transcriptase designed for one-step RT-qPCRRead more
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Catalog NumberQuantity
EP212B6B0032000 kU
EP212B6B00150 kU
EP212B6B002500 kU
Catalog number EP212B6B003
Price (CNY)/ Each
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Quantity:
2000 kU

Invitrogen Lyo-ready SuperScript III Flash Reverse Transcriptase joins the SuperScript product family as a reverse transcriptase designed for one-step RT-qPCR and point-of-care assay development. It is a novel SuperScript III Reverse Transcriptase variant, engineered for enhanced speed, thermostability, and inhibitor tolerance. Lyo-ready SuperScript III Flash Reverse Transcriptase provides enhanced sensitivity as low as five copies per reaction.

Benefits of Lyo-ready SuperScript III Flash Reverse Transcriptase include:

  • Excellent choice for one-step RT-qPCR
  • Fast cDNA synthesis (~1 minute) reduces the time to results
  • Hot-start enables high-throughput applications, benchtop stability, and improved specificity of one-step RT-qPCR
  • Thermostability up to 70°C allows elevated reaction temperatures and cDNA synthesis from complex RNA targets
  • High inhibitor tolerance makes the system less dependent on RNA sample purity
  • Commercial rights and customization enable product usage for commercial purposes

Hot start

Lyo-ready SuperScript III Flash Reverse Transcriptase is inactive at ambient temperatures due to the hot start mechanism, which enables room temperature reaction setup and improved specificity and consistency of RT-qPCR results. When used in conjunction with hot start DNA polymerase, such as Lyo-ready Platinum™ II Taq DNA Polymerase, a fully assembled master mix can be stored at room temperature for up to 24 hours, enabling high-throughput applications and compatibility with automation and point-of-care assay development. Due to enhanced specificity, this pair of enzymes also enables multiplex RT-qPCR of up to five targets.

Lyo-ready

Lyo-ready SuperScript III Flash Reverse Transcriptase is a glycerol-free enzyme that ensures compatibility with microfluidics, air-drying, and lyophilization technologies. For a full lyo-ready one-step RT-qPCR solution, we recommend using SuperScript III Flash Reverse Transcriptase together with Lyo-ready Platinum II Taq DNA polymerase. To learn more about Lyo-ready Enzymes for molecular diagnostics development visit the page thermofisher.cn/lyo-ready.  

OEM reverse transcriptase

This product includes commercial rights and broad customization capabilities. To learn more about OEM and raw materials for molecular diagnostics visit the page thermofisher.cn/mdx.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Detection MethodqPCR, RT-PCR
FormatStand-alone Enzyme
PCR Method1-step RT-qPCR
Reaction SpeedFast
Optimal Reaction Temperature60°C
Quantity2000 kU
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Virus Detection
Concentration60 U/μL
Hot StartBuild-In Hot Start
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Starting MaterialRNA
Unit SizeEach
Contents & Storage
Lyo-ready SuperScript™ III Flash Reverse Transcriptase, 2000 kU

Store at -20°C.