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Thermo Scientific™
Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
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| Catalog Number | No. of Reactions |
|---|---|
| F630XL | 2000 Reactions |
| F630L | 500 Reactions |
| F630S | 100 Reactions |
Catalog number F630XL
Price (CNY)/ Each
Price:10,040.00
Online offer:4,016.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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No. of Reactions:
2000 Reactions
Price (CNY)/ Each
Online offer:4,016.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Phusion™ Plus DNA Polymerase
Catalog numberF630XL
Price (CNY)/ Each
Online offer:4,016.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.
Features include:
- >100X higher sequence accuracy than Taq polymerase
- Universal annealing temperature of 60°C in PCR
- Hot-start mechanism reduces non-specific amplification and primer degradation
- Automation enabled due to 24-hour stability of assembled reactions
- High inhibitor tolerance for work with DNA of suboptimal purity
The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.
Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.
Applications
- High-fidelity PCR
- Cloning & mutagenesis
- Template generation for sequencing
- GC-rich PCR
- Long-range PCR (up to 20 kb)
- High-throughput PCR
Notes
- To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePhusion Plus DNA Polymerase
Reaction Speed15 to 30 sec./kb
Quantity2000 x 50 μL reactions
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)>100X
Hot StartBuilt-In Hot Start
No. of Reactions2000 Reactions
OverhangBlunt
Reaction FormatSeparate components
Size (Final Product)20 kb or less
Unit SizeEach
Contents & Storage
Four packs, each containing:
• Phusion Plus DNA Polymerase (250 μL)
• 5X Phusion Plus Buffer (5 x 1.25 mL)
• 5X Phusion GC Enhancer (4 x 1.25 mL)
Store at –15°C to –25°C.
• Phusion Plus DNA Polymerase (250 μL)
• 5X Phusion Plus Buffer (5 x 1.25 mL)
• 5X Phusion GC Enhancer (4 x 1.25 mL)
Store at –15°C to –25°C.