Falcon 4i Direct Electron Detector

The Thermo Scientific Falcon 4i Direct Electron Detector is the ideal solution for your single particle analysis and cryo tomography applications.

Find additional information here: Falcon 4i Direct Electron Detector

Key Benefits

• Best imaging quality The Falcon 4i Detector’s new, optimized chip design is based on the proven large pixel layout of the Falcon product range (14 μm), enhancing low-noise levels while improving electron localization for reduced coincidence noise. Additionally, it is fully integrated in Thermo Scientific EPU and Tomography Software. To further enhance productivity and ease of use, the camera comes with a new data management platform (DMP) which hosts EPU Data Management (powered by Thermo Scientific™ Athena™ Software), allowing easy organization of large datasets and streamlined interfacing with your image processing system. The new optional EPU Quality Monitor is an on-the-fly pre-processing tool (for motion correction and CTF estimation, including derived parameters) for evaluation of the acquired SPA data during the actual acquisition process. This allows users to judge the quality of the acquired data and optimize the data acquisition while it is happening. With its unique combination of highest image quality, high throughput and a streamlined solution for data management and quality monitoring, the Falcon 4i Detector enables the productivity boost required by today’s leading scientific and industrial communities.
• High throughput for enhanced productivity The 6x higher internal frame rate, in combination with improved electron event localization, yields exposure times that are reduced up to 10-fold as compared to the previous generation detector. In addition, for each exposure, significant times savings have been realized on overhead (dark image recording, offloading the data from the camera). Overall, this significantly boosts data throughput, leading to faster results and subsequently the need for less microscopy time. Additionally, the Falcon 4i Detector has a non-interfering, reactive dose-protection mechanism, which avoids the need for repetitive calibrations when changing optical settings. This is enabled by the improved frame rate and radiation hardness of the Falcon 4i Detector, causing it to be less prone to damage. Ultimately, the new dose protector concept facilitates an easier and faster experimental set-up.
• Ease of use The Falcon 4i Detector is fully integrated in the EPU and Tomography Software, allowing for smooth daily instrument operation and data acquisition. For single particle analysis, the hardware delivered with the Falcon camera now enables a data management and optional on-the-fly pre-processing solution. EPU Data Management Software comes pre-installed and facilitates the organization, viewing and sharing of single particle cryo-EM data. EPU streams data and metadata directly to a project, which is set up prior to the starting of an experiment. You can easily view all the project data and metadata at the microscope, or remotely through a secure connection, and comment or share it with your collaborators. Single particle analysis data acquisition can be further augmented with the optional EPU Quality Monitor which processes incoming EPU data on-the-fly. Drift correction and CTF estimation (including derived parameters such as defocus, phase shift, astigmatism, etc.) are visualized, allowing ongoing analysis of incoming image quality. Based on its results, you can optimize acquisition parameters and filter data sets according to quality indicators. In combination with the Falcon 4i Detector, these solutions help to generate high-quality results quickly, with confidence, and with the same ease of use EPU Software is known for.

Note: The Falcon 4i Direct Electron Detector is fully compatible with the Krios G4 Cryo-TEM and can also be retrofitted on existing Krios, Glacios, Talos Arctica and other 200 kV Talos TEMs.