FastDigest HindIII
FastDigest HindIII
Thermo Scientific™

FastDigest HindIII

5' A ↓A G C T T 3' 3' T T C G A ↑A 5' Thermo Scientific FastDigest HindIIIRead more
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Catalog NumberQuantity
FD05055 x 500 μL (2,500 Reactions)
FD0504800 μL (800 Reactions)
Catalog number FD0505
Price (CNY)/ 2,500 reactions
Price:1,441.00
Online offer:1,009.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
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Quantity:
5 x 500 μL (2,500 Reactions)
Request bulk or custom format
Price (CNY)/ 2,500 reactions
Online offer:1,009.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
Add to cart
FastDigest HindIII
Catalog numberFD0505
Price (CNY)/ 2,500 reactions
Online offer:1,009.00Web orders only. Excludes Supply Centers.
(ends 31-Dec-2024)
-
Add to cart
5'  A ↓A  G  C  T  T   3' 
3'  T  T  C  G  A ↑A   5' 

Thermo Scientific FastDigest HindIII restriction enzyme recognizes A^AGCTT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Thermo Scientific FastDigest HindIII is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Methylation SensitivityNot Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
EnzymeHind III
Compatible Buffer10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat InactivationYes
Optimal Reaction Temperature37°C
Type IIS RENo
Quantity5 x 500 μL (2,500 Reactions)
Product TypeRestriction Enzyme
Research CategoryTraditional Cloning
Product LineFastDigest™
Overhang5' protruding ends
Inactivation Temperature80°C
Unit Size2,500 reactions
Contents & Storage
• FastDigest HindIII (2,500 μL)
• 10X FastDigest Buffer (5 x 1.0 mL)
• 10X FastDigest Green Buffer (5 x 1.0 mL)

Store at –20°C.