Invitrogen™

iBlot™ 3 Western Blot Transfer Device

Catalog number: IB31001
Invitrogen™

iBlot™ 3 Western Blot Transfer Device

Catalog number: IB31001
Catalog Number
IB31001
Unit Size
1 device
Price (CNY)
Catalog NumberUnit SizePrice (CNY)
IB310011 deviceRequest A Quote
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The iBlot 3 Western Blot Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably in as few as three minutes and without the need for liquid buffers. The iBlot 3 system is compatible with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes and offers equivalent or better performance compared with traditional wet-transfer methods in a fraction of the time. The iBlot 3 Western Blot Transfer Device is an integral part of the iBlot dry blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

iBlot 3 Western Blot Transfer Device features include:
• Complete protein transfer in as few as three minutes
• High detection sensitivity and even, consistent protein transfer
• Two independently controlled transfer stations capable of transferring up to two midi or four mini blots
• Built-in adjustable cooling to ensure consistent transfer temperature
• Pre-programed transfer methods optimized for low, high, and broad molecular weight ranges
• Ability to create custom programs
• A simple, user-friendly interface

How the iBlot dry blotting system works
Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked filter papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen).

With the iBlot dry blotting system, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot 3 system, there is no need to prepare buffers or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Western Blot
For Use With (Equipment)
iBlot 3 Transfer Stacks
Gel Compatibility
Mini- or Midi-sized: Bis-Tris Gels, Tricine Gels, Tris-Acetate Gels, and Tris-Glycine Gels
Membrane Compatibility
Nitrocellulose, PVDF
Mode of Transfer
Dry
Product Line
iBlot 3
Running Time
∼3 to 10 min.
Throughput
2 Midi or 4 Mini Gels
Type
Transfer Device
Warranty
Standard, 1-year warranty included

Contents & Storage

• iBlot 3 Gel Transfer Device
• Western Blot Roller

Store at room temperature.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text