Biocemical Identification System (O.B.I.S.) PYR
Biocemical Identification System (O.B.I.S.) PYR
Thermo Scientific™

Biocemical Identification System (O.B.I.S.) PYR

Rapidly determine PYRase activity is streptococci and Citrobacter spp. with this biochemical identification system.

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Catalog number ID0580M
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Quantity:
60 Tests/Kit

Rapidly determine PYRase activity is streptococci and Citrobacter spp. with Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) PYR. This rapid colorimetric test delivers simple and rapid results for the determination of PYRase activity in streptococci and Citrobacter spp, offering a rapid diagnostic alternative to time-consuming culture methods. In addition, the same enzymatic activity may be used to aid differentiation of Salmonella spp. from Citrobacter spp and other Enterobacteriaceae1,2.

Use O.B.I.S. PYR as a rapid diagnostic alternative to time-consuming culture methods.

  • Simple—easy to implement
  • Rapid—results in 6 minutes of testing suspect colonies
  • Non-carcinogenic substrates

The O.B.I.S. - PYR test uses Test Cards impregnated with L-pyroglutamic acid 7-amino-4-methyl-coumarin (7AMC) and dimethylaminocinnamaldehyde for the detection of PYRase activity. The enzymatic hydrolysis of this substrate by enterococci, Group A streptococci and Citrobacter spp. produces a purple color following the addition of the Developing Solution3.

We have developed a new system for PYRase testing4 using a non-carcinogenic substrate in response to health concerns associated with the use of β-naphthylamide (a potent carcinogen)5. The PYRase area on the O.B.I.S. PYR test is impregnated with L-pyroglutamic acid 7-amino-4-methylcoumarin (7AMC)4. Dimethylaminocinnamaldehyde is used as a color development reagent. The enzymatic hydrolysis of the substrate produces a purple color on addition of the O.B.I.S. PYR Developing Solution4.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

General References:

  1. Chagla, A. H., Borczyk, A. A., Aldom, J. E., Dalla Rosa, S. and Cole, D. D. (1993). Evaluation of the L-Pyrrolidonyl-β-Napthylamide hydrolosis test for differentiation of members of the families Enterbacteriaceae and Vibrionaceae. J. Clin. Microbiol. 31. 1946-1948.
  2. Bennett, A. R., MacPhee, S., Betts, R. and Post, D. (1999). Use of enzyme pyrrolidonyl peptidase (Oxoid PYR test) to distinguish Citrobacter from Salmonella isolated from foods. Letters in Applied Microbiology. 28. 175-178
  3. Data on file at Oxoid
  4. Druggan, P., Roberts, P. and Swaine, D. (1999). A Rapid Chromogenic Method for the Differentiation of Citrobacter spp. and Salmonella spp. Directly from Enteric Media. Abstr. C-444 p71 Abstr. 99th Annual Meet. Am. Soc. Microbiol. 1999.
  5. Bascomb, S. and Manafi, M. (1998). Use of Enzyme tests in the Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci. Clinical Microbiological Reviews. 11. 318-340.
Specifications
DescriptionO.B.I.S. PYR
Detectable AnalytesPYRase activity
IncludesTest cards, developing solution, buffer solution
Quantity60 Tests/Kit
Unit SizeEach
Contents & Storage
2°C to 8°C